Metabolic Imaging & Tissue engineering Group News

Prof. D. Papkovsky and collaborators from Institute of Physiology (Essen, Germany) have contributed to the book chapter on the method of multi-parametric analysis of reactive oxygen species (ROS) in ex vivo culture of carotid body. This is the chapter from the book "Arterial chemoreceptors in physiology and pathophysiology" recently published by the Springer Publishing House. The full text can be found here .

Members of the lab Dr. A. Zhdanov, I. Okkelman and Prof. D. Papkovsky in collaboration with Prof. J. Cryan's lab from APC Microbiome Institute have published the research article devoted to study of intracellular oxygen (O2) gradients. While the existence of dynamic O2 gradients is a controversial topic, Zhdanov et al found that giant umbrella cells from mouse bladder epithelium display profound O2 gradients in ex vivo culture. Using confocal phosphorescence lifetime imaging microscopy (PLIM) and recently developed O2-sensitive Pt-Glc probe, they observed significant (up to 85 uM) lateral O2 gradients. Such gradients are presumably produced by mitochondrial respiration, as the authors demonstrated. The work was published in American Journal of Physiology - Cell Physiology. The full text can be found here . The image from the article was chosen as the "Image of the week" in the social media of the AJP Cell Physiology, on 14th of August.

On 20-24th of July Prof. D. Papkovsky has attended international symposium "Topical problems of biophotonics" (Volga river, Nizhny Novgorod, Russia). He presented a talk about imaging of hypoxia in 3D cultures of stem and cancer cells using phosphorescence-based nanosensors in section devoted to "Tumour and stem cells biology". More information on the event can be found here . Straight after this conference, Prof. D. Papkovsky and PhD candidate C. Kelly have attended the 9th Advanced Study Course on Optical Chemical Sensors (ASCOS) held in Porto, Portugal on 22-29th of July. Prof. D. Papkovsky delivered a talk on intracellular chemical sensing. Congratulations to Caroline, which team won the Project Group Work!