Metabolic Imaging & Tissue engineering Group News

Prof. R. Dmitriev will participate in online event 'See the hidden: discover more with FLIM' on 10-Oct-2024! More info & registration can be found here: https://events.bitesizebio.com/see-the-hidden-discover-more-with/join

Our team is growing and we can present at the same time in two different places now! PhD candidate Nore Van Loon (joint project with FunGen lab, Prof. S. Vergult) gave a talk at the Methods and Applications of Fluorescence 2024 conference (8-11 September Valencia, Spain)! She presented her work in progress on using autofluorescence and fluorescent cholera toxin labelling to monitor differentiation of neural cells in human iPSC-derived 'brain' organoids. This work is a part of ongoing efforts of bringing together multi-omics approaches and advanced imaging of organoids to better understand aetiology of such neurodevelopmental disorders as FOXG1 syndrome, funded by the Special Research Fund at Ghent University (BOF) and Marguerite-Marie Delacroix foundation. At the same time, Prof. Ruslan Dmitriev travelled to Munster, Germany, to present an invited lecture on imaging oxygenation and cell metabolism in multicellular spheroids in 4th Inflammation & Imaging symposium! This l...

Together with Prof. Margarida Barroso (Albany Medical College), we guest edit a collection of video protocols, hosted by the Journal of Visualised Experiments ( JoVE ), and  dedicated to the quantitative live cell imaging in 3D .  We expect bringing together some leaders and innovators in various aspects of the quantitative live fluorescence microscopies applied to ex vivo animal models, organoids and 3D engineered tissue blocks. The collection, which just has been started, can be assessed here .

Prof. R. Dmitriev will be presenting at HSR research symposium (Ghent University, 11-Feb-2021) and speak about new biosensors for organoid research. Full details and registration can be found here:  https://www.ugent.be/ge/hsr/en/news-events/events/2nd-hsr-research-symposium-2021

Our recent collaborative work on pH-sensing DAOTA dyes (published in Analytical Chemistry in 2019) has been included into the virtual issue of ACS Sensors journal, celebrating 5 years and entitled 'Giants in sensing'. To reflect back to what we have published over the period of 2015-2020 with ACS journals, see the list below: Dmitriev RI , Borisov SM, Düssmann H, Sun S, Müller BJ, Prehn J, Baklaushev VP, Klimant I, Papkovsky DB: Versatile Conjugated Polymer Nanoparticles for High-Resolution O2 Imaging in Cells and 3D Tissue Models.  ACS Nano  2015,  9 (5), 5275-5288. Dmitriev RI , O’Donnell N, Papkovsky DB:   Metallochelate Coupling of Phosphorescent Pt-Porphyrins to Peptides, Proteins, and Self-Assembling Protein Nanoparticles .   Bioconjugate Chemistry   2016,   27 (2):439-445. Fomin M,  Dmitriev RI , Jenkins J, Papkovsky DB, Heindl D, König B: A Two-Acceptor Cyanine-Based Fluorescent Indicator ...

Dr. Ruslan Dmitriev  recently presented a talk at the Biophysical Society 2020 Annual Meeting, in San Diego Convention Center, California, USA. In his talk, Ruslan summarised recent group developments in a multiparameter imaging of stem cell metabolism in the small intestinal organoid model. He focused on measuring oxygenation of live intestinal organoids, assessing mitochondrial polarisation and combining O2 with NAD(P)H redox microscopy analyses. He also received  Travel Award from the Biophysical Society to attend this meeting. More information on the meeting can be found on Twitter .  The abstract of presentation published in Biophysical Journal supplement can be viewed here .

During the ASCB-EMBO 2019 conference in Washington DC (December 2019) Dr. Ruslan Dmitriev was interviewed by Biotechniques journal about FLIM, organoids, challenges and career inspirations. Here's an interview: https://www.biotechniques.com/microscopy-imaging/ruslan-dmitriev-on-flim-and-intestinal-organoids/

The webpage of Sechenov First Medical University (Moscow, Russia) has highlighted our recent article on analysis of the mitochondrial membrane potential by FLIM method (published in autumn 2019). The media article (in Russian) can be found here .

Our Team will present at the American Society for Cell Biology / EMBO 2019 Annual Meeting! Dr. R. Dmitriev will give a short talk at the Microsymposium 17 (New perspectives in cell biology, Tuesday) entitled 'Multiplexed FLIM reveals dynamic stem cell niche metabolism in Lgr5-GFP intestinal organoids' and present a poster on the biosensor tissue engineering scaffold materials (P1629, Monday): These presentations will briefly summarise our main research efforts in designing novel imaging tools and their application in study of stem cell niche metabolism in organoids. R. Dmitriev has also received ASCB-EMBO postdoctoral Travel Award to attend this meeting. More info on this Meeting  and the  Program . Twitter

Our manuscript on comparing imaging methodologies (O2-PLIM vs. NAD(P)H-FLIM vs. Seahorse XF) with intestinal Lgr5-GFP organoids has been highlighted by preprint highlights service PreLights . Link

Dr. Irina Okkelman's work at the Irish Photonic Integration Centre (IPIC) / UCC has been acknowledged by the 'Best publication award in 2018' prize. For this contest, Irina submitted our recent work on the design of cellulose-based biosensor scaffolds for imaging of various tissue-engineered constructs ( O'Donnell et al, Acta Biomaterialia, 2018 ).  The award ceremony was held on 21-Mar-2019 in Aula Maxima, University College Cork.

Dr. R. Dmitriev organised, chaired and gave an invited talk at the Sechenov Biomedical Summit 2019 conference. His section 'Functional imaging in tissue engineering and regenerative medicine' gathered world-known experts in the areas of intestinal organoid and stem cell niche metabolism, non-invasive imaging in tissue engineering and development of novel fluorescent protein-based biosensors. Dr. Dmitriev's talk was on visualization of stem cell niche by the fluorescence lifetime imaging microscopy (FLIM). The details of the section can be found here: The conference website: https://sechenov-sibs.confreg.org

Dr. Ruslan Dmitriev will give a seminar at the Trinity College Dublin on December, 5th: The seminar will be hosted by Ass.-Prof. M. Monaghan. Twitter link .

Dr. Ruslan Dmitriev will co-chair and present at the Symposium 57 (57-SY) 'Multi-parametric quantitative imaging of live cells in 3D tissue models' during TERMIS-2018 World Congress. TERMIS World Congress is one of the major conferences in tissue engineering and regenerative medicine, gathering > 1,000 participants (~ 500 oral presentations) from around the world: https://www.termis.org/wc2018/general-information.php Dr. Dmitriev's section will gather some of the renown experts and cover the applications of FLIM, PLIM, fluorescence, Raman, X-Ray Phase contrast, ultrasound microscopy and multi-parametric imaging in tissue engineering. The section will be co-chaired with Prof. Y. Yang (Keele University, UK).

Prof. Klaus Suhling (King's College London) gave a seminar at IPIC/ Tyndall/ University College Cork on 12-June-2018 (academic host: Prof. Dmitri Papkovsky). Prof. Suhling is a renown expert in developing FLIM imaging devices and their successful application to study intracellular viscosity, lipids and other biomolecules. More on his research can be found here .

Prof. Dmitri Papkovsky and Dr. Ruslan Dmitriev edited the book, which summarises biological applications of quantitative O2 analysis by means of quenched-phosphorescence method. The book provides an excellent introduction to the topic, highlights its main applications, is written by experts and leaders in this research area and should be highly useful to the diverse community of specialists targeting the problems of cell bioenergetics, biomedical imaging, marine biology, photodynamic cancer therapy, food packaging and others. The book consists of 17 chapters (368 pages) and has been published by the Royal Society of Chemistry (series 'Detection Science'). More info can be found here . Twitter link Chapter 1 (Fundamentals, written by Prof. S. Borisov) is FREE to read!

Dr. R. Dmitriev gave a plenary (invited) talk at the Sechenov International Biomedical Summit (Moscow, Russia, 21-23 May), an emerging scientific symposium focusing on biomaterials, regenerative and personalized medicine. Dr. Dmitriev's talk was on applications of FLIM-PLIM microscopy to study the stem cell niche in the intestinal organoid model.  The conference program can be assessed here . The presentation can be viewed from here .

Members of the lab will attend the Europt(R)ode conference on 25-28 Mar 2018. They will discuss multi-parametric FLIM-PLIM imaging of 3D tissue models (Dr. R. Dmitriev, regular talk) and biomedical applications of phosphorescent oxygen sensors and probes (Prof. D. Papkovsky, invited talk). Europt(R)ode is one of the leading worldwide conferences focusing on innovations in optical sensors and biosensors and their multi-disciplinary applications. More information on the conference and the program can be found here .

Metabolic Imaging Group & Laboratory of Biophysics and Bioanalysis can be found on Twitter now! Make sure to follow our channel  @ PhotoBioLab  to stay informed about our latest accomplishments!

Prof. Dmitri Papkovsky and Dr. Ruslan Dmitriev have recently appeared in the news on a website of the School of Biochemistry/ University College Cork. Prof. Papkovsky's two-weeks lecture trip across Japanese universities, supported by the Japan Society for Promotion of Science has been mentioned here . Dr. Dmitriev has appeared in two news articles, mentioning his new book 'Multi-parametric live cell microscopy of 3D tissue models' published by Springer, and about his new role as ASCB ambassador .