Metabolic Imaging & Tissue engineering Group News

Dr. R. Dmitriev  performed and co-authored a collaborative study together with groups from Russia (Sechenov Institute for Regenerative Medicine and Privolzhsky Research Medical University) and Austria (TU Graz), which describes multi-parameter imaging of decellularized pericardial biomeshes in an implanted mouse model. One of highlights of the study is the finding that conjugated polymer-based O2 nanosensors can bind and remain bound to the biomeshes in live animal for extended period of time and thus used for subsequent O2 imaging in live animal and ex vivo. This confirms high usability of conjugated nanoparticle sensors previously developed by our team for such emerging applications as monitoring of tissue regeneration, tumour imaging and others. The work has been published in Frontiers in Bioengineering and Biotechnology journal. Full text can be assessed here . Twitter link .

Dr. Ruslan Dmitriev  recently presented a talk at the Biophysical Society 2020 Annual Meeting, in San Diego Convention Center, California, USA. In his talk, Ruslan summarised recent group developments in a multiparameter imaging of stem cell metabolism in the small intestinal organoid model. He focused on measuring oxygenation of live intestinal organoids, assessing mitochondrial polarisation and combining O2 with NAD(P)H redox microscopy analyses. He also received  Travel Award from the Biophysical Society to attend this meeting. More information on the meeting can be found on Twitter .  The abstract of presentation published in Biophysical Journal supplement can be viewed here .

During the ASCB-EMBO 2019 conference in Washington DC (December 2019) Dr. Ruslan Dmitriev was interviewed by Biotechniques journal about FLIM, organoids, challenges and career inspirations. Here's an interview: https://www.biotechniques.com/microscopy-imaging/ruslan-dmitriev-on-flim-and-intestinal-organoids/

Our manuscript on analysis of Lgr5-GFP intestinal organoid metabolism by different methods (O2-PLIM, NAD(P)H-FLIM and XF assay) has been accepted for publication by Redox Biology journal . This is an excellent result of collaboration with Prof. Michael Monaghan group at Trinity College Dublin (NAD(P)H-FLIM), which enabled us to compare and efficiently use 2 different FLIM-PLIM imaging platforms available in Ireland. Combining these innovative experimental approaches enabled us to discover highly 'flexible' and dynamic ability of stem cells to respond to the nutrient availability in the growth media. The next stages of our research on stem cell niche metabolism promise to be even more exciting! More on this story: Preprint Pre-Lights Twitter1 Twitter2  

The webpage of Sechenov First Medical University (Moscow, Russia) has highlighted our recent article on analysis of the mitochondrial membrane potential by FLIM method (published in autumn 2019). The media article (in Russian) can be found here .

In year 2019 we achieved the following: - 1 PhD student successfully graduated ( Neil O'Donnell , 2014-2018) - We published 5 research articles on new pH-sensitive ( Analytical Chemistry ), redox-sensitive ( Dyes and Pigments ) and O2 sensing films ( Sensors and Actuators B ), new FLIM method of visualising mitochondrial membrane potential ( Cytometry A ) and a work on tissue engineering ( Xenotransplantation ) - Submitted 1 preprint ( BioRxiv ) on assessing metabolism in the intestinal organoids, together with 2 other articles under consideration, 1 book chapter for the book 'Intestinal stem cells' (Springer, 2020?). - We presented at ISSCR Organoids Amsterdam Symposium (poster), Sechenov Biomedical Summit (Invited talk, organised and chaired a session), ASCB-EMBO 2019 (talk and poster), visited Leica training center and University Tubingen with short-term collaborative visits, lectures and seminars. - 3 funding awards were secured: SFI Industry Fello...

Our Team will present at the American Society for Cell Biology / EMBO 2019 Annual Meeting! Dr. R. Dmitriev will give a short talk at the Microsymposium 17 (New perspectives in cell biology, Tuesday) entitled 'Multiplexed FLIM reveals dynamic stem cell niche metabolism in Lgr5-GFP intestinal organoids' and present a poster on the biosensor tissue engineering scaffold materials (P1629, Monday): These presentations will briefly summarise our main research efforts in designing novel imaging tools and their application in study of stem cell niche metabolism in organoids. R. Dmitriev has also received ASCB-EMBO postdoctoral Travel Award to attend this meeting. More info on this Meeting  and the  Program . Twitter