Metabolic Imaging & Tissue engineering Group News

Dr. R. Dmitriev and Prof. D. Papkovsky are the authors of the new research article (communication) published in FEBS Letters journal (Dec. 2014). The paper entitled "In vitro ischemia decreases histone H4K16 acetylation in neural cells" reveals that oxygen and glucose deprivation of cultured cells (commonly used model of brain and heart ischemia) leads to transient decrease of acetylation status of histone H4 at residue K16 (H4K16). This epigenetic mark is involved in variety of cellular processes such as general chromatin decompaction or cell cycle arrest, if impaired, and is essential for survival of animal cell. This observation is expected to facilitate future progress in the area of stroke and help to develop better therapies. The full-text can be found here .

Dr. R. Dmitriev and Prof. D. Papkovsky have contributed to the book entitled "Neuronal Cell Death: Methods and Protocols" which is a part of widely known series "Methods in Molecular Biology" and will be published by Springer in early 2015. This book is dedicated to the novel and state-of-the-art cell and molecular biology methods and protocols of analysis of neuronal cell death and neurodegeneration with different biological models - from cells to in vivo. The chapter entitled " Multi-parametric O 2  Imaging in Three-Dimensional Neural Cell Models with the Phosphorescent Probes" describes detailed protocols on multi-parametric live cell imaging of spheroid (neurospheres) and brain slice ex vivo models and allowing to correlate their viability and cell death with oxygenation using high-resolution PLIM microscopy. The text of book chapter can be found here .