Neuronal cell death (book chapter)
Dr. R. Dmitriev and Prof. D. Papkovsky have contributed to the book entitled "Neuronal Cell Death: Methods and Protocols" which is a part of widely known series "Methods in Molecular Biology" and will be published by Springer in early 2015.
This book is dedicated to the novel and state-of-the-art cell and molecular biology methods and protocols of analysis of neuronal cell death and neurodegeneration with different biological models - from cells to in vivo.
The chapter entitled "Multi-parametric O2 Imaging in Three-Dimensional Neural Cell Models with the Phosphorescent Probes" describes detailed protocols on multi-parametric live cell imaging of spheroid (neurospheres) and brain slice ex vivo models and allowing to correlate their viability and cell death with oxygenation using high-resolution PLIM microscopy.
The text of book chapter can be found here.
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