Versatile Conjugated Polymer Nanoparticles for High-Resolution O2 Imaging in Cells and 3D Tissue Models
It is well-known that the fluorescent and phosphorescent nanoparticle sensor probes are a powerful imaging tool to study the physiology at the level of live cells and tissues. They are bright, exceptionally photostable and can be compatible with multiple detection modes - "multi-modal" probes - for quantitative measurements. However, they are often tailored to particular cell model and their targeting to specific cell types represents a challenging task.
To address this, Dr. S. Borisov (Graz University of Technology), Dr. R. Dmitriev and collaborators from Graz University of Technology (Austria), UCC, RCSI (Dublin) and Pirogov Russian State Medical University (Russia) introduced a new concept of copolymer nanoparticles for O2 sensing. These nanoparticles are based on two dyes (antenna and porphyrin) with controlled FRET leading to improved (5-10 times) brightness and two-photon absorption, in comparison to any existing Pt-porphyrin-based O2-sensitive nanoparticles. On the other hand, the copolymers can be prepared with different charged groups thus allowing their "optimisation" for particular biological models, which was demonstrated with cultured cells and multi-cellular tumour spheroid models. Such copolymer nanoparticles can become a useful tool for broad range of applications in biology and are expected to inspire the design of (bio) sensors for other analytes.
This work has been published in ACS Nano journal.
The full text of the article can be found here.
To address this, Dr. S. Borisov (Graz University of Technology), Dr. R. Dmitriev and collaborators from Graz University of Technology (Austria), UCC, RCSI (Dublin) and Pirogov Russian State Medical University (Russia) introduced a new concept of copolymer nanoparticles for O2 sensing. These nanoparticles are based on two dyes (antenna and porphyrin) with controlled FRET leading to improved (5-10 times) brightness and two-photon absorption, in comparison to any existing Pt-porphyrin-based O2-sensitive nanoparticles. On the other hand, the copolymers can be prepared with different charged groups thus allowing their "optimisation" for particular biological models, which was demonstrated with cultured cells and multi-cellular tumour spheroid models. Such copolymer nanoparticles can become a useful tool for broad range of applications in biology and are expected to inspire the design of (bio) sensors for other analytes.
This work has been published in ACS Nano journal.
The full text of the article can be found here.
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