Global translation response to brain ischemia, observed with neural PC12 cells
Dr. A. Zhdanov, Dr. R. Dmitriev and Prof. D. Papkovsky in collaboration with Dr. P. Baranov " LAPTI " group (University College Cork) and Moscow State University (Russia) have published joint research paper entitled "Oxygen and glucose deprivation induces widespread alterations in mRNA translation within 20 minutes" in Genome Biology open access journal. The team has focused efforts on novel technique - ribosome profiling - to determine immediate effects of cellular stress occurring during ischemia (oxygen and glucose deprivation) on global protein translation. Some of the key findings of the study are: -the indication of immediate cellular response, independent from well-known Hypoxia Inducible Factor (HIF) pathway; -short-list of the key genes which translation is changed upon ischemia and -widespread occurrence of non-canonical translation initiation that can lead to synthesis of "stress-induced" forms of proteins. Collectively, this data should...