Dr. R. Dmitriev, N. O'Donnell and Prof. D. Papkovsky published research in Bioconjugate Chemistry journal reporting new approach to construct phosphorescent O2-sensitive materials for biological imaging: they found that the metal chelation, widely used for immobilisation of proteins, is also compatible with the use of Pt-porphyrins for quantification of molecular oxygen. Team successfully demonstrated specific and non-covalent coupling of Pt-porphyrins to various types of biomolecules - peptides, proteins and self-assembling protein nanoparticles. After coupling, Pt-porphyrins retained their photophysical properties and were compatible with various measurement methods, such as microplate reader and fluorescence microscope. This study provides a framework for design of advanced protein- and peptide-based biosensors and should facilitate further progress in imaging tissue O2. The full text can be found here . The article is now part of special issue entitled ' Molecular imagi...
