Metallochelate Coupling of Phosphorescent Pt-Porphyrins to Peptides, Proteins, and Self-Assembling Protein Nanoparticles

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Dr. R. Dmitriev, N. O'Donnell and Prof. D. Papkovsky published research in Bioconjugate Chemistry journal reporting new approach to construct phosphorescent O2-sensitive materials for biological imaging: they found that the metal chelation, widely used for immobilisation of proteins, is also compatible with the use of Pt-porphyrins for quantification of molecular oxygen. Team successfully demonstrated specific and non-covalent coupling of Pt-porphyrins to various types of biomolecules - peptides, proteins and self-assembling protein nanoparticles. After coupling, Pt-porphyrins retained their photophysical properties and were compatible with various measurement methods, such as microplate reader and fluorescence microscope. This study provides a framework for design of advanced protein- and peptide-based biosensors and should facilitate further progress in imaging tissue O2.
The full text can be found here.
The article is now part of special issue entitled 'Molecular imaging probe chemistry'.

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