Live cell imaging of mouse intestinal organoids reveals heterogeneity in their oxygenation

By -

Okkelman et al, Biomaterials, 2017

Stem cells extracted from small intestine can grow in the culture dish, to produce mini-organ model termed organoids. They are promising for better understanding stem cell biology, developing drug therapies and success of regenerative medicine. The team led by Science Foundation Ireland (SFI)-funded Starting Investigator Dr. Ruslan Dmitriev studied the dynamics of oxygen supply in live  intestinal organoids using method of phosphorescence lifetime imaging microscopy (PLIM) and previously developed O2-sensitive small molecule conjugate. Dr. I. Okkelman, T. Foley, Prof. D. Papkovsky and Dr. R. Dmitriev found that organoids can display dramatic differences in their oxygenation and dynamic gradients, reflecting the heterogeneity of their metabolism and cellular composition. The work published in Biomaterials journal points at the importance of live microscopy analysis of engineered organoid tissue in overcoming the issues of heterogeneity, variability and data reproducibility.

The article text can be accessed here.
The free download link (expires on 31-Oct-2017): https://authors.elsevier.com/a/1ViXTWWN0almS  

Comments

Post a Comment

Popular posts from this blog

Affordable microscopy of spheroid oxygenation (video protocol)

By -
Image
Our lab has published a new protocol paper, this time with the video, in the Journal of Visualized Experiments (JoVE)! In this research method paper, we report the use of red/ near-infrared emitting biocompatible nanosensors to monitor oxygenation of the live multicellular spheroids (made of one or more cell types), together with the tracers of cell death. While this methodology is typically available only for users with 'high-end' PLIM microscopes and alike, we show that using of ratiometric readout with the red and near infrared emitting fluorescent nanosensors makes this possible on broadly available 'widefield' fluorescence microscope platforms. This method helps comparing oxygenation of spheroids, assessing this in a long-term and kinetic assays, comparing their variability and viability, essential in follow-up 3D printing applications. This is the first article in our collection ' Quantitative live cell imaging of 3D models' , guest-edited together with ...
Read more

JoVE Methods collection: Quantitative live cell imaging of 3D models

By -
Image
Together with Prof. Margarida Barroso (Albany Medical College), we guest edit a collection of video protocols, hosted by the Journal of Visualised Experiments ( JoVE ), and  dedicated to the quantitative live cell imaging in 3D .  We expect bringing together some leaders and innovators in various aspects of the quantitative live fluorescence microscopies applied to ex vivo animal models, organoids and 3D engineered tissue blocks. The collection, which just has been started, can be assessed here .
Read more