new horizons for FLIM research
In Jan-2019, Dr. Ruslan Dmitriev visited the Leica training Centre in Germany, where he tested the new platforms for confocal and multiphoton FLIM imaging, based on Leica Falcon SP8 system. In collaboration with Dr. H. Glauner, he tested the methods of labelling cell proliferation by FLIM, NADH autofluorscence FLIM and some other approaches with the culture of live intestinal organoids. White light laser- and multiphoton-based (Dive) Falcon SP8 microscopes were found to be excellent tools for studying three-dimensional complex structure of live intestinal organoids.
Pictured below: 3D reconstruction of FLIM images of live organoid with labeled proliferating and stem cells.
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