Metabolic Imaging & Tissue engineering Group News

Dr. R. Dmitriev will attend the Stem Cells & Organoids in Development and Disease symposium, where he will present a poster on the visualisation of stem cell niche by the multi-parametric FLIM approach. This international meeting promises to be one of the main events in the organoids science in 2019. More information and program can be found here . The program & abstracts are available  as PDF.

Neil O'Donnell (supervised by Dr. R. Dmitriev and Prof. D. Papkovsky ) has successfully defended his Ph.D. thesis on 22-Jan-2019! Neil's Ph.D. project ('New biosensors for metabolic imaging', 2014-2018) was funded by the Science Foundation Ireland (SFI) SIRG grant and focused on development, evaluation and application of biosensors, based on advanced coupling chemistry and various recombinant proteins. Neil participated in the following projects: 1. Metallochelate coupling of Pt-porphyrins to peptides, proteins and self-assembling protein nanoparticles for advanced biosensing applications. Published in Bioconjugate Chemistry journal. 2. pH-sensitive fluorescent protein-based nanoparticles for imaging of cancer and neural cells (unpublished). 3. Book chapter on the available biosensors for imaging of 3D tissue models. 4. Cellulose-binding domain fused with fluorescent proteins as a tool for design of hybrid 'biosensing' scaffolds for tissue engine...

In Jan-2019, Dr. Ruslan Dmitriev visited the Leica training Centre in Germany, where he tested the new platforms for confocal and multiphoton FLIM imaging, based on Leica Falcon SP8 system. In collaboration with Dr. H. Glauner, he tested the methods of labelling cell proliferation by FLIM, NADH autofluorscence FLIM and some other approaches with the culture of live intestinal organoids. White light laser- and multiphoton-based (Dive) Falcon SP8 microscopes were found to be  excellent tools for studying three-dimensional complex structure of live intestinal organoids. Pictured below: 3D reconstruction of FLIM images of live organoid with labeled proliferating and stem cells. Twitter link .

Dr. Ruslan Dmitriev recently collaborated with TU Graz and PCO AG, in research article targeting development of novel, highly photostable red fluorescent pH-sensitive dyes with extended fluorescence decay times (~7-12 ns). The team focused on the family of diazaoxotriangulenium dyes, as perspective biosensing materials for cell and environmental applications. The research was published in Analytical Chemistry journal. Twitter link.

Dr. Ruslan Dmitriev will give a seminar at the Trinity College Dublin on December, 5th: The seminar will be hosted by Ass.-Prof. M. Monaghan. Twitter link .

Neil O'Donnell , Dr. Irina Okkelman , Prof. Dmitri Papkovsky and Dr. Ruslan Dmitriev in collaboration with colleagues from the Institute for Regenerative Medicine, Russia have published new research article on the use of various cellulose-based scaffolds for advanced microscopy in  tissue engineering applications. The team has developed biosensor based on the recombinant fluorescent biosensing protein and fused with cellulose-binding domain: this cellulose-based pH-sensitive biosensor can label the decellularized plant tissue-derived scaffold (spinach, celery) and is useful for monitoring of extracellular acidification of the 3D culture of cancer cells and stem cell-derived intestinal organoids. Such biomaterial is also compatible with multiparametric FLIM-PLIM imaging of real-time oxygenation providing new framework for the label-free biomedical imaging of the tissue engineered in vitro and ex vivo . The research published in Acta Biomaterialia journal can be assessed...

Dr. Ruslan Dmitriev will co-chair and present at the Symposium 57 (57-SY) 'Multi-parametric quantitative imaging of live cells in 3D tissue models' during TERMIS-2018 World Congress. TERMIS World Congress is one of the major conferences in tissue engineering and regenerative medicine, gathering > 1,000 participants (~ 500 oral presentations) from around the world: https://www.termis.org/wc2018/general-information.php Dr. Dmitriev's section will gather some of the renown experts and cover the applications of FLIM, PLIM, fluorescence, Raman, X-Ray Phase contrast, ultrasound microscopy and multi-parametric imaging in tissue engineering. The section will be co-chaired with Prof. Y. Yang (Keele University, UK).