Hybrid scaffolds for O2 sensing in 3D cell cultures

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PhD candidate J. Jenkins, Dr. R. Dmitriev, Prof. D. Papkovsky and co-authors published joint research paper describing use of polymeric 3D scaffolds for mammalian cell culture for measurement of localised (pericellular) O2 levels with various cell models including cancer cells, multi-cellular aggregates and cultured brain slices. This method allows for better analysis of cell function and viability and can be combined with various other assays (study of cell differentiation) and pharmacological treatments (hypoxia-specific drugs), when performed on one-photon confocal PLIM microscope. The research is published in Acta Biomaterialia journal and can be found found here.

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