Metabolic Imaging & Tissue engineering Group News

Dr. A. Zhdanov, Dr. R. Dmitriev and Prof. D. Papkovsky in collaboration with Dr. P. Baranov " LAPTI " group (University College Cork) and Moscow State University (Russia) have published joint research paper entitled "Oxygen and glucose deprivation induces widespread alterations in mRNA translation within 20 minutes" in Genome Biology  open access journal. The team has focused efforts on novel technique - ribosome profiling - to determine immediate effects of cellular stress occurring during ischemia (oxygen and glucose deprivation) on global protein translation. Some of the key findings of the study are: -the indication of immediate cellular response, independent from well-known Hypoxia Inducible Factor (HIF) pathway; -short-list of the key genes which translation is changed upon ischemia and -widespread occurrence of non-canonical translation initiation that can lead to synthesis of "stress-induced" forms of proteins. Collectively, this data should

Dr. R. Dmitriev and Prof. D. Papkovsky have jointly co-authored review article in Methods and Applications in Fluorescence journal (IOP publications). The article provides short and concise overview of nearly all existing and optical luminescent probes suitable for quantification of intracellular oxygen in living cells. While the number of "novel O2 sensors" appears in the literature every year (at least 2-5 O2 probes per year), the critical analysis and cross-comparison between existing small molecule and nanoparticle sensor probes are normally missing. The review tries to perform classification among all the reported structures to answer such questions as "toxicity", "cell specificity", "best suitable biological model" etc. This review complements the previously published review articles on exhaustive classification of biological applications of quenched-phosphorescence O2 sensors and probes ( Papkovsky and Dmitriev, Chemical Society Review