O2 imaging of tumour spheroids helps to understand the function of Ca2+/Mn2+ pump

By -

PhD candidate J. Jenkins, Prof. D. Papkovsky and Dr. R. Dmitriev have published new research paper, demonstrating the application potential of multi-parametric O2 PLIM imaging. The multiplexed O2 and pH imaging of 3D tumour spheroid model helped to study the function of membrane-associated ATPase SPCA2, under the conditions close to physiological. Authors confirmed that the enzyme can act as Mn2+ pump, is dependent on cell density and environmental O2 and is regulated through reactive oxygen species and hypoxia-inducible factor (HIF) pathway. In addition, authors provide evidence that SPCA2 is involved in regulation of progression of cell cycle.
The work combining classical biochemical methods with modern quantitative imaging and tissue engineering approaches has been published in Biochemical Journal.
The full text can be found here.

Comments

Post a Comment

Popular posts from this blog

Affordable microscopy of spheroid oxygenation (video protocol)

By -
Image
Our lab has published a new protocol paper, this time with the video, in the Journal of Visualized Experiments (JoVE)! In this research method paper, we report the use of red/ near-infrared emitting biocompatible nanosensors to monitor oxygenation of the live multicellular spheroids (made of one or more cell types), together with the tracers of cell death. While this methodology is typically available only for users with 'high-end' PLIM microscopes and alike, we show that using of ratiometric readout with the red and near infrared emitting fluorescent nanosensors makes this possible on broadly available 'widefield' fluorescence microscope platforms. This method helps comparing oxygenation of spheroids, assessing this in a long-term and kinetic assays, comparing their variability and viability, essential in follow-up 3D printing applications. This is the first article in our collection ' Quantitative live cell imaging of 3D models' , guest-edited together with
Read more

JoVE Methods collection: Quantitative live cell imaging of 3D models

By -
Image
Together with Prof. Margarida Barroso (Albany Medical College), we guest edit a collection of video protocols, hosted by the Journal of Visualised Experiments ( JoVE ), and  dedicated to the quantitative live cell imaging in 3D .  We expect bringing together some leaders and innovators in various aspects of the quantitative live fluorescence microscopies applied to ex vivo animal models, organoids and 3D engineered tissue blocks. The collection, which just has been started, can be assessed here .
Read more