O2 imaging of tumour spheroids helps to understand the function of Ca2+/Mn2+ pump

By -

PhD candidate J. Jenkins, Prof. D. Papkovsky and Dr. R. Dmitriev have published new research paper, demonstrating the application potential of multi-parametric O2 PLIM imaging. The multiplexed O2 and pH imaging of 3D tumour spheroid model helped to study the function of membrane-associated ATPase SPCA2, under the conditions close to physiological. Authors confirmed that the enzyme can act as Mn2+ pump, is dependent on cell density and environmental O2 and is regulated through reactive oxygen species and hypoxia-inducible factor (HIF) pathway. In addition, authors provide evidence that SPCA2 is involved in regulation of progression of cell cycle.
The work combining classical biochemical methods with modern quantitative imaging and tissue engineering approaches has been published in Biochemical Journal.
The full text can be found here.

Comments

Post a Comment

Popular posts from this blog

Production and Multi-Parameter Live Cell Fluorescence Lifetime Imaging Microscopy (FLIM) of Multicellular Spheroids (JOVE protocol)

By -
Image
ALERT: New publication from our group! Angela, Gabriele, Hang, Nore, Nina and Irina teamed up to produce a video protocol paper, focusing on comparing various multicellular spheroid formation methods, suitable for 'downstream' live multi-parameter fluorescence lifetime imaging microscopy (FLIM) and imaging cell oxygenation. This protocol is illustrated with examples of autofluorescence-based FLIM microscopy of NAD(P)H, oxygenation and cell death, as well as imaging of visible flavin-based cell autofluorescence, performed in tumour and stem cell-derived spheroids. The article is also a part of the Methods collection 'Quantitative Live Cell Imaging of 3D Models', which is going to expand in the future.    Link to the publication: https://www.jove.com/t/66845/production-multi-parameter-live-cell-fluorescence-lifetime-imaging The Methods Collection (Journal of Visualised Experiments, JOVE):  https://app.jove.com/methods-collections/902
Read more

End of the Academic Year 2023-2024!

By -
Image
It's been busy, it's been tough but it's been also fun! Our team has trained a number of MSc students, with excellent projects, spanning the scope from establishment of novel organoid models, to testing of mechanical properties of the hydrogel-producing biomaterials on the small intestinal epithelium organoids and FLIM analysis of neural ('brain') iPSC-derived organoids. Thanks to everyone involved! The end of year (June) was culminated in presenting of research at Ghent Gut Inflammation Group (Ruslan, Irina, Hang, Lenie) and participating (as instructors, Ruslan and Michele) in the FEBS course on Functional Imaging of cellular dynamics (Amsterdam, 9-15 June 2024)!         Stay tuned for more exciting news on our research!
Read more

Conference presentations: September 2024

By -
Image
Our team is growing and we can present at the same time in two different places now! PhD candidate Nore Van Loon (joint project with FunGen lab, Prof. S. Vergult) gave a talk at the Methods and Applications of Fluorescence 2024 conference (8-11 September Valencia, Spain)! She presented her work in progress on using autofluorescence and fluorescent cholera toxin labelling to monitor differentiation of neural cells in human iPSC-derived 'brain' organoids. This work is a part of ongoing efforts of bringing together multi-omics approaches and advanced imaging of organoids to better understand aetiology of such neurodevelopmental disorders as FOXG1 syndrome, funded by the Special Research Fund at Ghent University (BOF) and Marguerite-Marie Delacroix foundation. At the same time, Prof. Ruslan Dmitriev travelled to Munster, Germany, to present an invited lecture on imaging oxygenation and cell metabolism in multicellular spheroids in 4th Inflammation & Imaging symposium! This l...
Read more