New publication: evaluation of GelMA as a Matrigel replacement for small intestinal organoids

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In our new study (collaboration between Ghent University and Rousselot BV) we report application of GelMA (gelatin methacryloyl) hydrogels with tuneable mechanical properties and added laminin to the culture of the small intestinal organoids. We found that mechanical properties alone are not sufficient for basement membrane support, resulting in polarity reversion, in contrast to the Matrigel-grown cultures. Altogether, observed polarity reversion phenomenon highlights importance of the further research in biochemical factors, directing cell polarity in the epithelial cells, while it also provides a tractable way for producing 'apical-out' organoids. Congratulations to Lenie (for her work on this MSc research project), Irina (for her supervision and driving force), Thomas and Ruben for expertise with gelatins and Bert for expertise with pig intestinal organoids.

The work has been published in the Journal of Tissue Engineering (Open Access): https://journals.sagepub.com/doi/10.1177/20417314251345000

 

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