Advanced Time-Correlated Single Photon Counting Applications (book chapter)

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PhD candidate James Jenkins, Dr. Ruslan Dmitriev and Prof. Dmitri Papkovsky have contributed to  the book "Advanced Time-Correlated Single Photon Counting Applications", recently published by Springer (series Chemical Physics, Volume 111, 2015). The book chapter entitled "Imaging cell and tissue O2 by TCSPC-PLIM" (pages 225-247) describes general protocols, methods and probes/ sensors suitable for real-time analysis of live cell and tissue oxygenation with broad variety of 2D and 3D models. TCSPC is a modern method allowing simultaneous measurements of both fluorescence and phosphorescence lifetimes of excited dyes. This can be applied for quantitative detection of O2, pH, Ca2+ and other physiological parameters.
The book chapter and the book can be accessed here.



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