Two-Acceptor Cyanine-Based Fluorescent Indicator for NAD(P)H in Tumor Cell Models

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Dr. R. Dmitriev, PhD candidate J. Jenkins and Prof. D. Papkovsky, in collaboration with Regensburg Institute of Organic Chemsitry and Roche Diagnostics (Germany), have developed a new fluorescent "turn-on" probe for NAD(P)H detection in cancer cells. Such red fluorescent probe works in "end-point" assay formats, however it is highly compatible with both 2D (adherent cell cultures) and 3D (tumour spheroid) cell models. The probe can be used for multi-parametric imaging of cell metabolism in advanced in vitro and ex vivo models of cancer.
The manuscript published in ACS Sensors journal can be found here.

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