Phosphorescence based O2 sensors – Essential tools for monitoring cell and tissue oxygenation and its impact on metabolism (tutorial review)

By -
Prof. D. Papkovsky and Dr. A. Zhdanov published a tutorial review dedicated to technical aspects of monitoring and keeping the control of O2 in cell culture, by optical methods. The review covers the macroscopical (environmental) control of O2 by solid-state sensors and possible readout modes (microplate readers, high-content screening platforms, microscopes) available to those wishing to know exact levels of hypoxia  experienced by the cells in culture. This complements other reviews recently published by the group, covering general aspects of O2 measurements by different methods (2012), the variety of biological applications where O2 measurements are needed (2013) and the problems and state-of-the-art of intracellular O2 probes (2015).
The review published in Free Radical Biology and Medicine journal can be assessed here.

Comments

Post a Comment

Popular posts from this blog

Affordable microscopy of spheroid oxygenation (video protocol)

By -
Image
Our lab has published a new protocol paper, this time with the video, in the Journal of Visualized Experiments (JoVE)! In this research method paper, we report the use of red/ near-infrared emitting biocompatible nanosensors to monitor oxygenation of the live multicellular spheroids (made of one or more cell types), together with the tracers of cell death. While this methodology is typically available only for users with 'high-end' PLIM microscopes and alike, we show that using of ratiometric readout with the red and near infrared emitting fluorescent nanosensors makes this possible on broadly available 'widefield' fluorescence microscope platforms. This method helps comparing oxygenation of spheroids, assessing this in a long-term and kinetic assays, comparing their variability and viability, essential in follow-up 3D printing applications. This is the first article in our collection ' Quantitative live cell imaging of 3D models' , guest-edited together with
Read more

JoVE Methods collection: Quantitative live cell imaging of 3D models

By -
Image
Together with Prof. Margarida Barroso (Albany Medical College), we guest edit a collection of video protocols, hosted by the Journal of Visualised Experiments ( JoVE ), and  dedicated to the quantitative live cell imaging in 3D .  We expect bringing together some leaders and innovators in various aspects of the quantitative live fluorescence microscopies applied to ex vivo animal models, organoids and 3D engineered tissue blocks. The collection, which just has been started, can be assessed here .
Read more