Phosphorescence based O2 sensors – Essential tools for monitoring cell and tissue oxygenation and its impact on metabolism (tutorial review)

By -
Prof. D. Papkovsky and Dr. A. Zhdanov published a tutorial review dedicated to technical aspects of monitoring and keeping the control of O2 in cell culture, by optical methods. The review covers the macroscopical (environmental) control of O2 by solid-state sensors and possible readout modes (microplate readers, high-content screening platforms, microscopes) available to those wishing to know exact levels of hypoxia  experienced by the cells in culture. This complements other reviews recently published by the group, covering general aspects of O2 measurements by different methods (2012), the variety of biological applications where O2 measurements are needed (2013) and the problems and state-of-the-art of intracellular O2 probes (2015).
The review published in Free Radical Biology and Medicine journal can be assessed here.

Comments

Post a Comment

Popular posts from this blog

New synthetic hydrogels for tumour spheroids and intestinal organoid cultures (publication)

By -
Image
  We collaborated with groups of Prof. Richard Hoogenboom and Prof. Devriendt (Ghent University) and tested application of poly(2-alkyl-2-oxazoline) hydrogels (PAOx) for 3D cell cultures of cancer and stem cells. We found that encapsulation of small intestinal organoids into PAOx hydrogels results in polarity reversion, i.e. transition from basal to apical-out phenotype. This is useful for studying drug delivery, nutrient absorption and host-microbiota interactions within this model.  This figure shows dramatic differences of mitochondrial polarisation (TMRM) and appearance (transmission light) in small intestinal organoids, observed after apical-out transition (PAOx), in contrast to conventional Matrigel-grown culture: Since the PAOx hydrogels can show variable mechanical properties, we tested if encapsulation into stiff and soft hydrogels can affect behaviour of cancer cells in 3D: indeed, with HCT116 spheroids we found that stiffness of the hydrogels affect cell migration f...
Read more

Production and Multi-Parameter Live Cell Fluorescence Lifetime Imaging Microscopy (FLIM) of Multicellular Spheroids (JOVE protocol)

By -
Image
ALERT: New publication from our group! Angela, Gabriele, Hang, Nore, Nina and Irina teamed up to produce a video protocol paper, focusing on comparing various multicellular spheroid formation methods, suitable for 'downstream' live multi-parameter fluorescence lifetime imaging microscopy (FLIM) and imaging cell oxygenation. This protocol is illustrated with examples of autofluorescence-based FLIM microscopy of NAD(P)H, oxygenation and cell death, as well as imaging of visible flavin-based cell autofluorescence, performed in tumour and stem cell-derived spheroids. The article is also a part of the Methods collection 'Quantitative Live Cell Imaging of 3D Models', which is going to expand in the future.    Link to the publication: https://www.jove.com/t/66845/production-multi-parameter-live-cell-fluorescence-lifetime-imaging The Methods Collection (Journal of Visualised Experiments, JOVE):  https://app.jove.com/methods-collections/902
Read more

Happy New Year 2025! New publications.

By -
Image
Wow, time really does fly! It's been a while since our previous update but by no means our team was idle.  End of 2024 was memorable with exciting conferences, including SPAOM 2024, Histochemical Society Annual online meeting 2024, FHERITALE consortium and others! We've received funding to study neurodevelopmental and neurodegenerative diseases (more news soon) so we can plan new & exciting research projects. Our team currently hosts 2 international researchers, Valerie Cote and Dr. Simone Perottoni, both learning and doing exciting science in FLIM, organoids and imaging of cancer cells / tissues. In addition, we are looking forward to host more of PhD students from FLIMagin3D consortium! Here are some recent publications, from our team and collaborative research projects: Sheehan A, Okkelman IA, Groslambert G, Bucher C, Dmitriev RI, Filatov MA: Optoelectronic Properties and Fluorescence Lifetime Imaging Application of Donor-Acceptor Dyads Derived From 2,6-DicarboxyBODIPY ...
Read more