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Metabolic Imaging Group & Laboratory of Biophysics and Bioanalysis can be found on Twitter now!
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Production and Multi-Parameter Live Cell Fluorescence Lifetime Imaging Microscopy (FLIM) of Multicellular Spheroids (JOVE protocol)

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ALERT: New publication from our group! Angela, Gabriele, Hang, Nore, Nina and Irina teamed up to produce a video protocol paper, focusing on comparing various multicellular spheroid formation methods, suitable for 'downstream' live multi-parameter fluorescence lifetime imaging microscopy (FLIM) and imaging cell oxygenation. This protocol is illustrated with examples of autofluorescence-based FLIM microscopy of NAD(P)H, oxygenation and cell death, as well as imaging of visible flavin-based cell autofluorescence, performed in tumour and stem cell-derived spheroids. The article is also a part of the Methods collection 'Quantitative Live Cell Imaging of 3D Models', which is going to expand in the future.    Link to the publication: https://www.jove.com/t/66845/production-multi-parameter-live-cell-fluorescence-lifetime-imaging The Methods Collection (Journal of Visualised Experiments, JOVE):  https://app.jove.com/methods-collections/902
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End of the Academic Year 2023-2024!

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It's been busy, it's been tough but it's been also fun! Our team has trained a number of MSc students, with excellent projects, spanning the scope from establishment of novel organoid models, to testing of mechanical properties of the hydrogel-producing biomaterials on the small intestinal epithelium organoids and FLIM analysis of neural ('brain') iPSC-derived organoids. Thanks to everyone involved! The end of year (June) was culminated in presenting of research at Ghent Gut Inflammation Group (Ruslan, Irina, Hang, Lenie) and participating (as instructors, Ruslan and Michele) in the FEBS course on Functional Imaging of cellular dynamics (Amsterdam, 9-15 June 2024)!         Stay tuned for more exciting news on our research!
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New synthetic hydrogels for tumour spheroids and intestinal organoid cultures (publication)

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  We collaborated with groups of Prof. Richard Hoogenboom and Prof. Devriendt (Ghent University) and tested application of poly(2-alkyl-2-oxazoline) hydrogels (PAOx) for 3D cell cultures of cancer and stem cells. We found that encapsulation of small intestinal organoids into PAOx hydrogels results in polarity reversion, i.e. transition from basal to apical-out phenotype. This is useful for studying drug delivery, nutrient absorption and host-microbiota interactions within this model.  This figure shows dramatic differences of mitochondrial polarisation (TMRM) and appearance (transmission light) in small intestinal organoids, observed after apical-out transition (PAOx), in contrast to conventional Matrigel-grown culture: Since the PAOx hydrogels can show variable mechanical properties, we tested if encapsulation into stiff and soft hydrogels can affect behaviour of cancer cells in 3D: indeed, with HCT116 spheroids we found that stiffness of the hydrogels affect cell migration f...
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