new horizons for FLIM research

By -

In Jan-2019, Dr. Ruslan Dmitriev visited the Leica training Centre in Germany, where he tested the new platforms for confocal and multiphoton FLIM imaging, based on Leica Falcon SP8 system. In collaboration with Dr. H. Glauner, he tested the methods of labelling cell proliferation by FLIM, NADH autofluorscence FLIM and some other approaches with the culture of live intestinal organoids. White light laser- and multiphoton-based (Dive) Falcon SP8 microscopes were found to be  excellent tools for studying three-dimensional complex structure of live intestinal organoids.

Pictured below: 3D reconstruction of FLIM images of live organoid with labeled proliferating and stem cells.


Twitter link.

Comments

Post a Comment

Popular posts from this blog

Affordable microscopy of spheroid oxygenation (video protocol)

By -
Image
Our lab has published a new protocol paper, this time with the video, in the Journal of Visualized Experiments (JoVE)! In this research method paper, we report the use of red/ near-infrared emitting biocompatible nanosensors to monitor oxygenation of the live multicellular spheroids (made of one or more cell types), together with the tracers of cell death. While this methodology is typically available only for users with 'high-end' PLIM microscopes and alike, we show that using of ratiometric readout with the red and near infrared emitting fluorescent nanosensors makes this possible on broadly available 'widefield' fluorescence microscope platforms. This method helps comparing oxygenation of spheroids, assessing this in a long-term and kinetic assays, comparing their variability and viability, essential in follow-up 3D printing applications. This is the first article in our collection ' Quantitative live cell imaging of 3D models' , guest-edited together with
Read more

JoVE Methods collection: Quantitative live cell imaging of 3D models

By -
Image
Together with Prof. Margarida Barroso (Albany Medical College), we guest edit a collection of video protocols, hosted by the Journal of Visualised Experiments ( JoVE ), and  dedicated to the quantitative live cell imaging in 3D .  We expect bringing together some leaders and innovators in various aspects of the quantitative live fluorescence microscopies applied to ex vivo animal models, organoids and 3D engineered tissue blocks. The collection, which just has been started, can be assessed here .
Read more