A new preprint from the lab!

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Our team has completed research project focusing on comparison of different methodologies to assess cell bionergetics in the intestinal organoid culture. In order to do that, we analysed Lgr5-GFP mouse organoids (courtesy of Prof. H. Clevers group, Hubrecht Institute) with previously established O2-PLIM method, microplate reader-based Agilent XF96 assay and two-photon excited NAD(P)H-FLIM, in collaboration with Prof. M. Monaghan's group (Trinity College Dublin). For the first time, we found that oxygenation heterogeneity of organoids has functional meaning and is dependent on the metabolic function of stem cell niche. We also observed that stem cells grown in organoids can quickly respond to changes in glucose content in the growth medium.
The current preprint version of the manuscript can be found on BioRxiv

(picture above: NADH-FLIM image of live Lgr5-GFP organoid produced on two-photon FLIM microscope Leica Dive SP8 Falcon. Different colors reflect fluorescence lifetimes and differences in cell metabolism).

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