Intestinal stem cells book

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Our team has contributed to the Intestinal Stem Cells book, edited by Prof. P. Ordonez-Moran and published by Springer. The long-awaited book has gathered a number of experts and pioneers in the fields of intestinal stem cell biology, organoids, tissue engineering and innovative methodologies to study them.

Our book chapter, written in collaboration with Jens Puschhof (Prof. Hans Clevers lab) describes  applications of FLIM and 3D FLIM for visualising stem cell niche in the small intestinal organoids. We describe imaging of oxygenation, cell and redox metabolism, labelling cell proliferation, with particular attention to 3D FLIM and multiplexed detection.
The chapter link.
Twitter link

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